Molecular Diagnostics / PCR
Comprehensive quantitative PCR and RT-PCR assays for viral, bacterial and metazoan pathogens of rodent, ruminant, and aquatic animals. Assays can be developed as novel pathogens are discovered (Mouse Kidney Parvovirus or Murine Chapparvovirus (MuCPV)) or outbreaks occur.
Optimal sample is kidney (pink curve). Fresh feces (blue curve) or a cage swab (yellow curve) can be submitted but are less sensitive samples.
Test |
|
Recommended Sample Type(s) |
Rate code |
|---|---|---|---|
| Rodent pathogens | |||
| Corynebacterium bovis | fur/skin swab, skin, environmental/cage swab | B070 | |
| Helicobacter (Species, h. hepaticus, h. bilis, h. rodentium, h. typhlonius) | cecal wall, feces (up to five fecal pellets can be pooled), environmental swab | B020 | |
| Mycoplasma sp. (All species) | cell culture, lung, oral swab, abscess swab | B060 | |
| Pseudomonas aeruginosa | cecal contents, feces, environmental swab | B070 | |
| Encephalitozoon cuniculi | feces, kidney | B070 | |
| Fur Mites (Myocoptes musculinis, Radfordia/Myobia sp.) | fur swab, environmental/cage swab | B090 | |
| Pinworms (Aspiculuris tetraptera, Syphacia obvelata, Syphacia muris) | feces, environmental/cage swab | B070 | |
| Pneumocystis carinii/murina | lung | B040 | |
| Mouse hepatitis virus (MHV) | feces, liver | B130 | |
| Mouse kidney parvovirus (MKPV) | kidney, feces, urine | B120 | |
| Mouse norovirus (MNV) | feces, environmental swab | B080 | |
| Mouse parvovirus 1-7 (MPV) | mesenteric lymph node, feces, spleen | B110 | |
| Zoonotic pathogens | |||
| Q fever (Coxiella burnettii) | amniotic fluid, placenta, uterus, whole blood (EDTA tube) | B050 | |
| Aquatic animal pathogens | |||
| Edwardsiella ictaluri | whole fish, head | B070 | |
| Ichthyophthirius multifiliis | whole fish, head | B070 | |
| Mycobacterium (Species, M. chelonae, H. haemophilum, M. marinum) | whole fish, gut | B070 |
Other assays and assay development services are available. Please inquire.
Submissions
Please submit samples in a 1.5ml microcentrifuge tube when possible. The seal-rite tubes are preferable since they have a tighter seal. If submitting large samples, a 15 or a 50ml conical tube will suffice. If submitting feces or swabs, up to 5 fecal pellets or 5 swabs can be pooled into one tube. Ship samples to the lab priority overnight via FedEx or UPS. Samples should be shipped in an insulated container with ice packs. Please contact the lab with any questions.
Preferred Sample Collection TubesUSA Scientific: Seal-Rite® 1.5 mL Microcentrifuge Tubes: #1615-2500 |
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